HA-CDC25B overexpression induces the formation of extra-numerary centrin 2 “foci”.

<p>Asynchronous U2OS-HA-CDC25B cells were incubated in the continued presence (+ tet) or absence (- tet) of tetracycline as in Figure 1, fixed 48 hours later and co-stained for HA-CDC25B (red), centrin 2 (green) and DAPI (blue). (A) Examples of centrin 2 foci in cells expressing (- tet, arrows) HA-CDC25B in comparison to control cells (+ tet). Bar = 10 µm. (B) Histogram plot showing the percentage of cells harbouring excess centrin 2 “foci” following 48 hours HA-CDC25B expression. Bars represent means of at least 200 cells counted from three independent experiments +/- SD.